Reduction of Trimethylene Dipyridyl with Illuminated Chloroplasts.

Effects of intermediates of the photosynthetic carbon reduction cycle on carbon metabolism in spinach chloroplasts illuminated on filter paper discs.

Carbon dioxide and the reduction of indophenol and ferricyanide by chloroplasts.

Pea chloroplasts isolated in salt media show decreased rates of 2:6 dichlorophenolindophenol (DCPIP) and ferricyanide reduction when depleted of CO(2) at pH values below 7.5. The greatest effect of CO(2) was on uncoupled systems. The incorporation of 10(-2), 2 x 10(-2) and 4 x 10(-2)m sodium acetate into the reaction mixtures progressively increased the bicarbonate concentration required for ha...

Enzymic Mechanisms of Pyridine Nucleotide Reduction in Chloroplasts.

The photochemical reduction of triphosphopyridine nucleotide by isolated chloroplasts has been known since 1951 (see “Discussion”). The discovery of this photochemical reaction marked a significant advance in the biochemical analysis of the energy conversion process of photosynthesis because it established the first link between photon capture by chloroplast pigments and the reduction of a phys...

Redox Reactions between Kaempferol and Illuminated Chloroplasts.

Bleaching of kaempferol by illuminated chloroplasts was observed at 380 nanometers. The photobleaching was stimulated by methyl viologen and suppressed by superoxide dismutase indicating the participation of O(2) (-) in the reaction. An electron transfer inhibitor on the oxidizing side of photosystem II, carbonylcyanide m-chlorophenylhydrazone (CCCP), stimulated the photobleaching and 3-(3,4-di...

Studies on Plastoquinonez-1. Determination of the Concentration and Oxidation-reduction State of Plastoquinone in Isolated Chloroplasts

It was shown by Cranes that plastoquinone is localized in the chloroplasts of higher plants, and there is now a good deal of evidence which suggests that it bctions as an oxidation-reduction carrier in the photosynthetic electron transport system. Bishop’ showed that when lyophilized chloroplasts were extracted with organic solvents they lost their Hill-reaction activity, but that this could be...